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Journal of Pharmaceutical and BioSciences

 
Original Article
Year : 2017   |  Volume : 5  |  Issue : 2  |  Page : 14-19

Stability indicating reproducible high performance liquid chromatographic method for determination of losartan potassium and atenolol in bulk and tablet dosage forms

P. S. Tungenwar, Sufiyan Ahmad, V. M. Shastry, Tabrej Mujawar
An accurate, precise and reproducible high-performance liquid chromatographic (RP-HPLC) method was developed for quantitative estimation of losartan potassium and atenolol simultaneously in tablet dosage forms. Young Lin (S.K.) gradient system ultraviolet detector and RP C18 (Thermo) with 250 mm × 4.6 mm i.d. and 5 μm particle size. Methanol 0.1% O-phosphoric acid (65:35) was used as the mobile phase for the method. The detection wavelength was 274 nm, and flow rate was 0.9 ml/min. In the developed method, the retention time of losartan potassium and atenolol was found to be 7.76 min and 4.05 min, respectively. The drug was subjected to oxidation, acid hydrolysis, alkaline hydrolysis, and heat to apply stress condition for degradation. The method was validated for specificity, linearity, precision, accuracy, robustness, and solution stability. The linearity, precision, range, and robustness were within the limits as specified by the International Conference on Harmonization Guidelines. Hence, the method was found to be simple, accurate, precise, economic, and reproducible. Hence, the proposed methods can be used for the routine quality control analysis of losartan potassium and atenolol in bulk drug as well as in formulations.
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