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Year : 2017   |  Volume : 5  |  Issue : 1  |  Page : 1-5

Development and Validation of Stability Indicating Rp-Hplc Method for Simultaneous Estimation of Mefenamic Acid and Dicyclomine Hydrochloride in Bulk and in Pharmaceutical Solid Dosage Form
A simple and sensitive stability indicating reversed phase High Performance Liquid Chromatography method was developed and validated for the simultaneous estimation of Mefenamic acid (MEF) and Dicyclomine hydrochloride (DCL) in bulk and in pharmaceutical solid dosage form. The column GLS-ODS C18 (250 x 4.6mm i.d. 5µm) having isocratic mode with mobile phase containing methanol was used. The flow rate was 1mL/min and the wavelength of detection was 263nm. The linearity of MEF and DCL were in the range of 25-125µg/ml and 100-500µg/ml respectively. The retention time of MEF and DCL were found to be 2.27min and 7.41min respectively. The percentage recoveries were found 99.999% and 99.993% of MEF and DCL respectively. The developed method was found to be accurate, precise and selective for simultaneous estimation of MEF and DCL in pharmaceutical solid dosage form (Tablet).
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