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Journal of Pharmaceutical and BioSciences

 
Original Article
Year : 2017   |  Volume : 5  |  Issue : 2  |  Page : 20-22

Qualitative and quantitative characterization of phytoconstituents from stem bark of Stereospermum colais Buch

Mohammed Imran, Yogesh Sonawane, Rashid Akhtar, Sufiyan Ahmed
Upon the qualitative analysis work on Stereospermum colais stem bark belongs to family Bignoniaceae, the phytochemical constituents such as steroids, carbohydrates, triterpenoids, phenolic compounds, coumarins, flavonoids, saponins, anthraquinones, proteins, and lipids were identified in stem bark of S. colais. Quantitative analysis of phenols, flavonoids, and triterpenoids was further performed. The active phytoconstituent was analyzed using thin-layer chromatography (TLC) and preliminary phytochemical screening of stem bark; from the methanolic extracts of stem bark showed various phytoconstituents such as flavonoids, phytosterols, saponins, and coumarins. Some important inorganic elements such as iron, chloride, potassium, phosphate, nitrate, and sulfate were identified by total ash analysis additionally. The quantitative determination of total phenolic content (72.5 mg/g), total flavonoid content (70.53 mg/g), and total triterpenoid content (12.68 mg/g) was performed using the same extract. In addition, for further purification of identified chemical constituents, TLC was performed and it showed remarkable results. The methanolic extract of S. colais stem bark contains betasitosterol and lupeol which are very useful therapeutically mostly to treat diabetes and heart diseases.
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