Induction and Establishment of Hairy Root Culture of Solanum xanthocarpum using Agrobacterium rhizogenes

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DOI: 10.4103/2231-4040.197331

Hairy Root Culture is the new route for large scale secondary metabolite production because of their fast and plagiotropic growth, genetic and biochemical stability. In this report Solanum xanthocarpum was used to induce the hairy root cultures, for getting the benefits of the medicinal properties residing in the roots of the medicinally important plant. About 65.38% survivability was obtained for S. xanthocarpum axillary buds when cultured on MS medium supplemented with IBA (0.25mg/l), FAP (0.5mg/l) and 1% activated charcoal for explants preparation. As, a result of an effective infection of Agrobacterium rhizogenes carrying Ri plasmid with rolC gene, hairy root cultures were established, which were grown in culture media without hormones. The Agrobacterium rhizogenes MTCC532, strain used in the present study was capable of inducing hairy roots formation by simple stabbing method with high transformation frequency (75%) after 12 days of infection. Copious growth of Hairy Roots was observed in ½ MS liquid medium without hormones with growth index of 45 with doubling time of 7 days demonstrated a simple and effective method of establishment of hairy root cultures. PCR analysis showed amplification of the rolC gene in Hairy Roots, indicated the transformation event. Further, this study allows future scale-up of hairy roots in bioreactors for large scale production of secondary metabolites and increasing use of this as model system in metabolic engineering,

Keywords: Hairy Root Culture, Solanum xanthocarpum, Agrobacterium rhizogenes, Secondary Metabolites.