Development and validation of HPLC-UV method for estimation of swertiamarin in Enicostemma littorale

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DOI: 10.4103/2231-4040.197331

Secoiridoid glycosides are a group of phytoconstituents present in Gentianaceae family and are known for their antidiabetic and hypolipedimic activities. Swertiamarin is the major physiologically active secoiridoid glycoside reported in Enicostemma littorale Blume (Gentianaceae). This study is designed for development and validation of an HPLC-UV method for estimation of swertiamarin. The developed HPLC-UV method was found accurate and reproducible for the detection and quantification of swertiamarin. A well-resolved peak of swertiamarin appeared at Rt 3.51±0.07 min with methanol-water (80:20) as mobile phase. The linear regression analysis data for the calibration curve showed good linearity in the concentration range of 100-1000 µg/ml with a correlation coefficient (r2) of 0.9994. The linear regression equation was y=6310.5x+869205. The limit of detection and limit of quantitation were found to be about ?19 and ?58 µg/ml, respectively. The method was found to be accurate (98.09-100.70 % recovery), precise (< 2 % R.S. 1.5 % R.S.D.). The proposed method was employed for the estimation of swertiamarin in E. littorale. Statistical analysis proved that the method was precise, selective and accurate for the estimation of swertiamarin.

Keywords: Swertiamarin, Enicostemma littorale, Gentianaceae, HPLC, ICH guidelines