The present work describes development and validation by high-performance liquid chromatography by UV detector procedure for the analysis of hydrochlorothiazide (HCZ) and Nebivolol (NBL) in pharmaceutical mixture. Effective chromatographic separation of HCZ and NBL was achieved using a Youngline (S.K.) Gradient System UV detector. Reverse phase (GRACE) C18 column, (4.6 mm × 250 mm; 5 μm), an SP930D pump, a 20 μl injection loop, and UV 730D absorbance detector and running Autochro-3000 software. Mobile phase composed of 0.1% solution of orthophosphoric acid in water and methanol in the proportion of (40:60), respectively. The flow rate is 0.7 ml/min on detecting wavelength 282 nm. The PH of mobile phase was 3.2. The proposed high-performance liquid chromatography method was statistically validated with respect to linearity, ranges, precision, accuracy, selectivity, limit of detection, limit of quantitation and robustness. The retention time of HCZ and NBL and were found to be 4.6833 and 9.18333, respectively. All parameters were found to be within the acceptance limit. The calibration curves were linear in ranges of 25-125 and 10-50, μg/ml respectively for HCZ and NBL. The R2 of HCZ and NBL was found to be 0.999, 0.999, respectively.

DOI: 10.31555/jpbs/2017/5/3/23-27

Films were prepared using 5 parts of 10% w/w of mucilage of gum of Cordia dichotoma with different proportions of plasticizers: Polyethylene glycol (PEG 400) (0.1, 0.15, 0.2, and 0.3), glycerin (0.15), and propylene glycol (0.2). The films were casted on glass plates and dried under controlled evaporation. Films prepared with 0.15, 0.2 part of PEG 400; 0.15 part of glycerin and propylene glycol showed satisfactory drying after 24 h. They were evaluated for following parameters water uptake, tensile strength, folding endurance, and water vapor transmission rate. The results obtained are comparable with films made from other polymers, and the gum can be used for preparing polymeric drug delivery systems and as film coating agent.

DOI: 10.31555/jpbs/2017/5/3/28-29