To study detail pharmacognostical profile of stem bark of Stereospermum colais. Assessment of pharmacognostical micro and macroscopical characters of fresh and dried stem bark samples were analyzed. WHO recommended parameter was utilized for investigation of physiochemical studies and fluorescent characters of the stem bark samples were also tested. The stem bark is woody shrub aboriginal to Asian country. It is straight stemmed deciduous tree 18-30 m in height 2.8 m in girth. Stem bark shows fractures on its outer surface and inner side shows light yellowish shade. Stem bark shows layer of epidermis, underneath this lies 2-4 rows of parenchyma with rounded or arc-shaped vascular bundles, lignified tissue, pericyclic non-lignified fibers, mucilage cells and 5-6 rows of longitudinal and 5-6 rows of tangential stone cells. The results of the study can carry as a valuable source of information and provide suitable standards for identification of this plant material in future investigations and applications.

DOI: 10.31555/jpbs/2017/5/2/10-13

An accurate, precise and reproducible high-performance liquid chromatographic (RP-HPLC) method was developed for quantitative estimation of losartan potassium and atenolol simultaneously in tablet dosage forms. Young Lin (S.K.) gradient system ultraviolet detector and RP C18 (Thermo) with 250 mm × 4.6 mm i.d. and 5 μm particle size. Methanol 0.1% O-phosphoric acid (65:35) was used as the mobile phase for the method. The detection wavelength was 274 nm, and flow rate was 0.9 ml/min. In the developed method, the retention time of losartan potassium and atenolol was found to be 7.76 min and 4.05 min, respectively. The drug was subjected to oxidation, acid hydrolysis, alkaline hydrolysis, and heat to apply stress condition for degradation. The method was validated for specificity, linearity, precision, accuracy, robustness, and solution stability. The linearity, precision, range, and robustness were within the limits as specified by the International Conference on Harmonization Guidelines. Hence, the method was found to be simple, accurate, precise, economic, and reproducible. Hence, the proposed methods can be used for the routine quality control analysis of losartan potassium and atenolol in bulk drug as well as in formulations.

DOI: 10.31555/jpbs/2017/5/2/14-19

Upon the qualitative analysis work on Stereospermum colais stem bark belongs to family Bignoniaceae, the phytochemical constituents such as steroids, carbohydrates, triterpenoids, phenolic compounds, coumarins, flavonoids, saponins, anthraquinones, proteins, and lipids were identified in stem bark of S. colais. Quantitative analysis of phenols, flavonoids, and triterpenoids was further performed. The active phytoconstituent was analyzed using thin-layer chromatography (TLC) and preliminary phytochemical screening of stem bark; from the methanolic extracts of stem bark showed various phytoconstituents such as flavonoids, phytosterols, saponins, and coumarins. Some important inorganic elements such as iron, chloride, potassium, phosphate, nitrate, and sulfate were identified by total ash analysis additionally. The quantitative determination of total phenolic content (72.5 mg/g), total flavonoid content (70.53 mg/g), and total triterpenoid content (12.68 mg/g) was performed using the same extract. In addition, for further purification of identified chemical constituents, TLC was performed and it showed remarkable results. The methanolic extract of S. colais stem bark contains betasitosterol and lupeol which are very useful therapeutically mostly to treat diabetes and heart diseases.

DOI: 10.31555/jpbs/2017/5/2/20-22